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The identification of rat intervertebral CEP cells

Journal: Chinese Journal of Reparative and Reconstructive Surgery

Article Title: 白藜芦醇对炎症所致椎间软骨终板退变中高迁移率蛋白1信号的影响

doi: 10.7507/1002-1892.202110084

Figure Lengend Snippet: The identification of rat intervertebral CEP cells

Article Snippet: Methods The intervertebral CEP cells of Sprague Dawley (SD) rats aged 3 weeks were extracted and identified by toluidine blue staining and immunofluorescence staining of rabbit anti-rat collagen type II.

Techniques:

The effect of RES on the activity of intervertebral CEP cells detected by CCK-8 assay

Journal: Chinese Journal of Reparative and Reconstructive Surgery

Article Title: 白藜芦醇对炎症所致椎间软骨终板退变中高迁移率蛋白1信号的影响

doi: 10.7507/1002-1892.202110084

Figure Lengend Snippet: The effect of RES on the activity of intervertebral CEP cells detected by CCK-8 assay

Article Snippet: Methods The intervertebral CEP cells of Sprague Dawley (SD) rats aged 3 weeks were extracted and identified by toluidine blue staining and immunofluorescence staining of rabbit anti-rat collagen type II.

Techniques: Activity Assay, CCK-8 Assay

The detection of targets of RES on rat intervertebral CEP cells

Journal: Chinese Journal of Reparative and Reconstructive Surgery

Article Title: 白藜芦醇对炎症所致椎间软骨终板退变中高迁移率蛋白1信号的影响

doi: 10.7507/1002-1892.202110084

Figure Lengend Snippet: The detection of targets of RES on rat intervertebral CEP cells

Article Snippet: Methods The intervertebral CEP cells of Sprague Dawley (SD) rats aged 3 weeks were extracted and identified by toluidine blue staining and immunofluorescence staining of rabbit anti-rat collagen type II.

Techniques:

Evaluation of the apoptosis of CEP cells following LiCl treatment. (A) Representative graphs of flow cytometric analysis. (B) Apoptotic rate of CEP cells was significantly higher following LiCl treatment, whereas inhibiting Wnt/β-catenin signalling protected against cellular apoptosis. The results are presented as the percentage of cell apoptosis. *P<0.05 CEP-CTR vs. CEP-NOM and CEP-INB. CEP-NOM, cells cultured under normal conditions; CEP-CTR, cells treated with LiCl; CEP-INB, cells treated with LiCl and a β-catenin inhibitor; CEP, cartilage endplate; LiCl, lithium chloride; FITC, fluorescein isothiocyanate.

Journal: Molecular Medicine Reports

Article Title: β-catenin signalling inhibits cartilage endplate chondrocyte homeostasis in vitro

doi: 10.3892/mmr.2019.10301

Figure Lengend Snippet: Evaluation of the apoptosis of CEP cells following LiCl treatment. (A) Representative graphs of flow cytometric analysis. (B) Apoptotic rate of CEP cells was significantly higher following LiCl treatment, whereas inhibiting Wnt/β-catenin signalling protected against cellular apoptosis. The results are presented as the percentage of cell apoptosis. *P<0.05 CEP-CTR vs. CEP-NOM and CEP-INB. CEP-NOM, cells cultured under normal conditions; CEP-CTR, cells treated with LiCl; CEP-INB, cells treated with LiCl and a β-catenin inhibitor; CEP, cartilage endplate; LiCl, lithium chloride; FITC, fluorescein isothiocyanate.

Article Snippet: The proliferation of CEP cells was assessed at 0, 24, 48 and 72 h with a Cell Counting Kit-8 (CCK-8) assay (Beyotime Institute of Biotechnology).

Techniques: Cell Culture